| CPC A01N 1/0252 (2013.01) [A61M 2205/3606 (2013.01); A61M 2206/10 (2013.01)] | 18 Claims |

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1. A method for freezing an individual bag or a plurality of bags containing biological fluid, favoring bottom-up ice-growth, comprising the following steps:
providing a controlled temperature chamber; placing an air flow system into the controlled temperature chamber; placing a bag or a case containing the bag on a support; compressing the bag or case against the support; driving air inside the controlled temperature chamber to obtain a differential air flow for freezing the bag, wherein the differential air flow is obtained by blowing air on the bottom of the bag or case such that a velocity of the air on the bottom of the bag or case is higher than a velocity of the air on the top of the bag or case, allowing bottom-up ice-growth; wherein a heat transfer coefficient on the top of the bag is less than 5 W/(m2° C.) and a heat transfer coefficient on the bottom of the bag is higher than 20 W/(m2° C.), and wherein the air flow has a velocity in a range from 1 m/s to 10 m/s.
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