US 11,946,932 B2
Integrated microfluidic system for automated multiplex analyte detection
Yuchao Chen, Rodeo, CA (US)
Assigned to WellSIM Biomedical Technologies, Inc., San Jose, CA (US)
Filed by WellSIM Biomedical Technologies, Inc., San Jose, CA (US)
Filed on May 31, 2023, as Appl. No. 18/326,021.
Claims priority of provisional application 63/347,549, filed on May 31, 2022.
Prior Publication US 2023/0384303 A1, Nov. 30, 2023
Int. Cl. G01N 33/543 (2006.01); B01L 3/00 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/54386 (2013.01) [B01L 3/502761 (2013.01); G01N 33/6896 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0654 (2013.01); B01L 2400/0487 (2013.01); G01N 2333/4709 (2013.01); G01N 2800/2821 (2013.01)] 23 Claims
OG exemplary drawing
 
1. A microfluidic device comprising:
(a) a reagent layer, said reagent layer comprising:
a sample reservoir for loading a sample solution,
a washing buffer reservoir for depositing a washing buffer solution,
a detection probe reservoir for depositing a detection probe solution, and
a waste reservoir for collecting a waste liquid;
(b) a microfluidic layer beneath the reagent layer, said microfluidic layer comprising:
a sample zone for receiving a first liquid from the sample reservoir,
a washing buffer zone for receiving a second liquid from the washing buffer reservoir,
a detection probe zone for receiving a third liquid from the detection probe reservoir, and
at least one reaction zone,
wherein the at least one reaction zone is connected with and allow an inflow of the first liquid from the sample zone, the second liquid from the washing buffer zone and the third liquid from the detection probe zone, and wherein the at least one reaction zone is connected with and allow an outflow of the waste liquid from the at least one reaction zone to the waste reservoir;
(c) a pierceable isolation layer spaced between the reagent layer and the microfluidic layer, wherein the pierceable isolation layer blocks the first, second and third liquid flow from the sample reservoir, the washing buffer reservoir, and the detection probe reservoir to the sample zone, the washing buffer zone, and the detection probe zone, respectively; and
(d) a substrate layer beneath the microfluidic layer.