CPC G01N 30/06 (2013.01) [G01N 30/46 (2013.01); G01N 30/88 (2013.01); G01N 2030/065 (2013.01); G01N 30/89 (2013.01)] | 17 Claims |
1. An analysis system comprising:
an autosampler;
a multidimensional chromatography system including a supercritical chromatography column and two reverse phase liquid chromatography columns, a nonpolar substance being eluted and removed in the supercritical chromatography column, and a weakly polar substance being adsorbed to a stationary phase of the supercritical chromatography column and further transported to the two reverse phase liquid chromatography columns;
a column oven in which a column is to be provided;
a liquid pump configured to transport a modifier to the supercritical chromatography column and transport a mobile phase to the two reverse phase liquid chromatography columns; and
a mass spectrometer, wherein:
the two reverse phase liquid chromatography columns include a first reverse phase liquid chromatography column and a second reverse phase liquid chromatography column;
the weakly polar substance adsorbed to the supercritical chromatography column is transported to the first reverse phase liquid chromatography column by only an action of the modifier to remove the supercritical mobile phase flowing out of the supercritical chromatography column in the first reverse phase liquid chromatography column; and
the weakly polar substance is transported to the second reverse phase liquid chromatography column to separate a component.
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