US 11,946,911 B2
Analysis system and method for rapidly detecting vitamin d in an oil or fat, or a biological sample
Yanli Guo, Beijing (CN); Naoki Hamada, Beijing (CN); and Yuki Hashi, Beijing (CN)
Assigned to SHIMADZU CORPORATION, Kyoto (JP)
Appl. No. 17/049,249
Filed by SHIMADZU CORPORATION, Kyoto (JP)
PCT Filed Jan. 25, 2019, PCT No. PCT/JP2019/002587
§ 371(c)(1), (2) Date Oct. 20, 2020,
PCT Pub. No. WO2019/207870, PCT Pub. Date Oct. 31, 2019.
Claims priority of application No. 201810394669.1 (CN), filed on Apr. 27, 2018.
Prior Publication US 2021/0239656 A1, Aug. 5, 2021
Int. Cl. G01N 30/46 (2006.01); G01N 30/06 (2006.01); G01N 30/88 (2006.01); G01N 30/89 (2006.01)
CPC G01N 30/06 (2013.01) [G01N 30/46 (2013.01); G01N 30/88 (2013.01); G01N 2030/065 (2013.01); G01N 30/89 (2013.01)] 17 Claims
OG exemplary drawing
 
1. An analysis system comprising:
an autosampler;
a multidimensional chromatography system including a supercritical chromatography column and two reverse phase liquid chromatography columns, a nonpolar substance being eluted and removed in the supercritical chromatography column, and a weakly polar substance being adsorbed to a stationary phase of the supercritical chromatography column and further transported to the two reverse phase liquid chromatography columns;
a column oven in which a column is to be provided;
a liquid pump configured to transport a modifier to the supercritical chromatography column and transport a mobile phase to the two reverse phase liquid chromatography columns; and
a mass spectrometer, wherein:
the two reverse phase liquid chromatography columns include a first reverse phase liquid chromatography column and a second reverse phase liquid chromatography column;
the weakly polar substance adsorbed to the supercritical chromatography column is transported to the first reverse phase liquid chromatography column by only an action of the modifier to remove the supercritical mobile phase flowing out of the supercritical chromatography column in the first reverse phase liquid chromatography column; and
the weakly polar substance is transported to the second reverse phase liquid chromatography column to separate a component.