US 11,946,909 B2
Microfluidic asymmetric flow field-flow fractionation device and method of using the same
Hugo Salmon, Paris (FR); Rabah Gahoual, Paris (FR); Nathalie Mignet, Clamart (FR); and Pascal Houze, Beauchamp (FR)
Assigned to Assistance Publique-Hopitaux de Paris, (FR); Centre National de la Recherche Scientifique (CNRS), (FR); Institut National de le Recherche Scientifique (CNRS), (FR); UNIVERSITÉ PARIS CITÉ, (FR); and Ecole Nationale Superieure de Chimie de Paris, (FR)
Appl. No. 16/971,763
Filed by Assistance Publique-Hopitaux de Paris, Paris (FR); Centre National de la Recherche Scientifique (CNRS), Paris (FR); Institut National de la Sante et de la Recherche Medicale (INSERM), Paris (FR); Universite De Paris, Paris (FR); and Ecole Nationale Superieure de Chimie de Paris, Paris (FR)
PCT Filed Feb. 21, 2019, PCT No. PCT/EP2019/054365
§ 371(c)(1), (2) Date Aug. 21, 2020,
PCT Pub. No. WO2019/162404, PCT Pub. Date Aug. 29, 2019.
Claims priority of application No. 18305185 (EP), filed on Feb. 22, 2018.
Prior Publication US 2021/0088483 A1, Mar. 25, 2021
Int. Cl. G01N 30/00 (2006.01)
CPC G01N 30/0005 (2013.01) [G01N 2030/003 (2013.01)] 17 Claims
OG exemplary drawing
 
1. An asymmetric flow field-flow fractionation device configured to separate particles dispersed in a liquid mobile phase, the device including:
a fractionation microchannel comprising a sample inlet and a sample outlet,
an auxiliary microchannel comprising an auxiliary outlet, and
a semipermeable membrane separating the fractionation microchannel and the auxiliary microchannel, said membrane being permeable to liquid and being configured to maintain the particles in said fractionation microchannel,
the fractionation microchannel being superimposed on the auxiliary microchannel, wherein the device comprises two layers, each layer containing a microfabricated recess having a thickness of less than 100 μm, the membrane being mechanically held by encapsulation in between the two layers, the recesses respectively defining the fractionation microchannel and the auxiliary microchannel on each side of the membrane.