CPC C12Q 1/6886 (2013.01) [C12N 9/22 (2013.01); C12N 15/1065 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/683 (2013.01); C12N 2800/80 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/154 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/166 (2013.01)] | 29 Claims |
1. A method for analyzing nucleic acid molecules in a biological sample, comprising:
a) physically partitioning at least a subset of the nucleic acid molecules in the biological sample based on methylation status of the nucleic acid molecules into a plurality of partitioned sets, wherein the biological sample comprises methylated nucleic acid molecules and unmethylated nucleic acid molecules; the plurality of partitioned sets comprises a first partitioned set and a second partitioned set and the methylated nucleic acid molecules are overrepresented in the first partitioned set relative to the second partitioned set;
b) digesting the first partitioned set with at least one methylation sensitive restriction enzyme, thereby removing incorrectly partitioned molecules; and
c) sequencing at least a subset of the nucleic acid molecules of the first partitioned set.
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