US 11,946,103 B2
Cleavable disulfide linkers and uses thereof
Ronald Graham, Carlsbad, CA (US); and Megha Cila, San Dliego, CA (US)
Assigned to Singular Genomics Systems, Inc., San Diego, CA (US)
Filed by Singular Genomics Systems, Inc, San Diego, CA (US)
Filed on Aug. 14, 2023, as Appl. No. 18/449,601.
Application 18/449,601 is a continuation of application No. PCT/US2022/052357, filed on Dec. 9, 2022.
Claims priority of provisional application 63/311,607, filed on Feb. 18, 2022.
Claims priority of provisional application 63/301,775, filed on Jan. 21, 2022.
Claims priority of provisional application 63/288,369, filed on Dec. 10, 2021.
Prior Publication US 2023/0407389 A1, Dec. 21, 2023
Int. Cl. C12Q 1/68 (2018.01); C07H 19/10 (2006.01); C07H 19/20 (2006.01); C12Q 1/6874 (2018.01); C12Q 1/6876 (2018.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6874 (2013.01) [C07H 19/10 (2013.01); C07H 19/20 (2013.01); C12Q 1/6876 (2013.01); C12Q 1/6806 (2013.01)] 20 Claims
 
1. A compound having the formula:

OG Complex Work Unit Chemistry
wherein
B is a divalent nucleobase;
R1 is a polyphosphate moiety, 5′-nucleoside protecting group, monophosphate moiety, or nucleic acid moiety;
R2 is hydrogen or —OH;
R3 is a reversible terminator moiety, hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
R4 is a detectable moiety;
L100 is a divalent linker comprising

OG Complex Work Unit Chemistry
 wherein
R5 and R6 are independently unsubstituted alkyl, —CN, —OH, —NH2, —COOH, —CONH2, —NO2, —SO3H, —SO4H, —SO2NH2, —NHNH2, —ONH2, substituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl; and
W1 and W2 are —O—.