US 11,946,102 B2
Coupling method
James Anthony Clarke, Oxford (GB); James White, Oxford (GB); John Milton, Oxford (GB); and Clive Gavin Brown, Cambridge (GB)
Assigned to Oxford Nanopore Technologies PLC, Oxford (GB)
Filed by Oxford Nanopore Technologies PLC, Oxford (GB)
Filed on Oct. 7, 2020, as Appl. No. 17/064,628.
Application 17/064,628 is a continuation of application No. 16/428,845, filed on May 31, 2019, granted, now 11,136,623.
Application 16/428,845 is a continuation of application No. 16/243,118, filed on Jan. 9, 2019, granted, now 11,041,194.
Application 16/243,118 is a continuation of application No. 14/122,573, granted, now 10,246,741, previously published as PCT/GB2012/051191, filed on May 25, 2012.
Claims priority of provisional application 61/599,246, filed on Feb. 15, 2012.
Claims priority of provisional application 61/490,860, filed on May 27, 2011.
Prior Publication US 2021/0087623 A1, Mar. 25, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6869 (2018.01); B82Y 15/00 (2011.01); G01N 27/447 (2006.01); G01N 33/487 (2006.01)
CPC C12Q 1/6869 (2013.01) [B82Y 15/00 (2013.01); G01N 27/44743 (2013.01); G01N 33/48721 (2013.01)] 23 Claims
OG exemplary drawing
 
1. A method for detecting analytes, comprising:
(a) providing a membrane in which is present a nanopore that provides a channel through the membrane;
(b) contacting the membrane, in an ionic solution, with one or more analytes, wherein following contact with the membrane the analytes are tethered to the membrane via a tethering group;
(c) applying a potential difference across the membrane and detecting a first analyte using the nanopore, from among the analytes tethered to the membrane; and
(d) detecting a second analyte, from among the analytes tethered to the membrane, using the same nanopore, wherein the second analyte is not the first analyte;
wherein the one or more analytes is an amino acid, peptide, polypeptide, or protein.