	US 11,946,092 B2
	Method for producing 2-keto-3-deoxygluconate from 2-(acetylamino)-2-deoxy-d-gluconic acid by two enzymes
Yuzhong Zhang, Jinan (CN); Pingyi Li, Jinan (CN); Wenxin Jiang, Jinan (CN); Xiulan Chen, Jinan (CN); Yishuo Zhang, Jinan (CN); and Xiaoyan Song, Jinan (CN)
Assigned to SHANDONG UNIVERSITY, Jinan (CN)
Filed by SHANDONG UNIVERSITY, Jinan (CN)
Filed on Oct. 31, 2022, as Appl. No. 18/051,024.
Prior Publication US 2023/0323409 A1, Oct. 12, 2023
Int. Cl. C12P 7/42 (2006.01); C12N 9/78 (2006.01); C12N 15/70 (2006.01)

CPC C12P 7/42 (2013.01) [C12N 9/78 (2013.01); C12N 15/70 (2013.01); C12Y 305/01 (2013.01); C12Y 305/04 (2013.01); C12N 2800/101 (2013.01)]

6 Claims

1. A method for producing 2-keto-3-deoxygluconate (KDG) from 2-(acetylamino)-2-deoxy-D-gluconic acid (GlcNAc1A), wherein said method comprises contacting GlcNAc1A with a deacetylase having the amino acid sequence of SEQ ID NO: 3 and a deaminase having the amino acid sequence of SEQ ID NO: 4 for 1-24 h to generate KDG.