US 11,946,083 B2
Method for the microbial production of specific natural capsaicinoids
Hui Chen, North Billerica, MA (US); Xiaodan Yu, Lexington, MA (US); LanLan Zhou, Bedford, MA (US); Hongxue Wang, Jiangsu (CN); and Min Wang, Bedford, MA (US)
Assigned to Conagen Inc., Bedford, MA (US)
Filed by Conagen Inc., Bedford, MA (US)
Filed on Aug. 24, 2022, as Appl. No. 17/821,810.
Application 17/821,810 is a division of application No. 16/317,844, granted, now 11,459,591, previously published as PCT/US2017/042944, filed on Jul. 19, 2017.
Claims priority of provisional application 62/363,951, filed on Jul. 19, 2016.
Prior Publication US 2022/0403427 A1, Dec. 22, 2022
Int. Cl. C12P 13/02 (2006.01); A23L 27/10 (2016.01); A61K 9/00 (2006.01); C07C 233/18 (2006.01); C07C 233/20 (2006.01); C11B 9/00 (2006.01)
CPC C12P 13/02 (2013.01) [A23L 27/10 (2016.08); A61K 9/0014 (2013.01); C07C 233/18 (2013.01); C07C 233/20 (2013.01); C11B 9/0061 (2013.01); C12Y 203/01086 (2013.01); A23V 2002/00 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A biosynthetic method of making a glucoside of a capsaicinoid of interest comprising:
a) expressing an acyl-CoA synthetase (ACS) and capsaicin synthase (CS) in a transformed cellular system;
b) expressing a UDP-glucosyltransferase in the transformed cellular system, wherein the UDP-glucosyltransferase is a CaUGT2 from C. roseus and either is encoded by a DNA sequence at least 75% identical to SEQ ID NO: 5 and/or comprises an amino acid sequence at least 90% identical to SEQ ID NO: 6;
c) growing the cellular system in a medium; and,
d) producing the glucoside of a capsaicinoid of interest.