	US 11,946,081 B2
	Bacillus gibsonii-clade serine proteases
Lilia Maria Babe, Emerald Hills, CA (US); Lydia Dankmeyer, Rotterdam (NL); Frits Goedegebuur, Vlaardingen (NL); Thijs Kaper, Half Moon Bay, CA (US); Harm Mulder, Voorhout (NL); and Sina Pricelius, Leiden (NL)
Assigned to Danisco US Inc., Palo Alto, CA (US)
Appl. No. 16/471,686
Filed by DANISCO US INC., Palo Alto, CA (US)
PCT Filed Dec. 19, 2017, PCT No. PCT/US2017/067356 § 371(c)(1), (2) Date Jun. 20, 2019, PCT Pub. No. WO2018/118950, PCT Pub. Date Jun. 28, 2018.
Claims priority of provisional application 62/437,509, filed on Dec. 21, 2016.
Prior Publication US 2019/0330610 A1, Oct. 31, 2019
Int. Cl. C12N 9/54 (2006.01); C11D 3/386 (2006.01)

CPC C12N 9/54 (2013.01) [C11D 3/38681 (2013.01); C12Y 304/21062 (2013.01)]

9 Claims

1. A subtilisin variant comprising an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the variant has the substitutions 5039E-5099R-S126A-D127E-F128G numbered by correspondence with the amino acid sequence of SEQ ID NO:1, and wherein the variant has a blood/milk/ink on cotton (BMI), egg, and/or crème brûlée stain cleaning PI>1 when compared to SEQ ID NO:1.