	US 11,946,075 B2
	Freeze dried viral nanoparticle constructs
Jonathan Pokorski, San Diego, CA (US); Nicole Steinmetz, San Diego, CA (US); and Parker Lee, Los Angeles, CA (US)
Assigned to CASE WESTERN RESERVE UNIVERSITY, Cleveland, OH (US)
Filed by CASE WESTERN RESERVE UNIVERSITY, Cleveland, OH (US)
Filed on Jul. 19, 2022, as Appl. No. 17/868,144.
Application 17/868,144 is a continuation of application No. 16/859,400, filed on Apr. 27, 2020, granted, now 11,390,853.
Claims priority of provisional application 62/839,438, filed on Apr. 26, 2019.
Prior Publication US 2023/0174952 A1, Jun. 8, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 7/00 (2006.01)

CPC C12N 7/00 (2013.01) [C12N 2770/18023 (2013.01); C12N 2770/18051 (2013.01); C12N 2770/32023 (2013.01); C12N 2770/32051 (2013.01); C12Y 301/27005 (2013.01)]

19 Claims

1. A method of producing plant virus-like particles, the method comprising:

freeze drying an aqueous solution of plant virus particles to produce substantially RNA-free plant virus-like particles, wherein the plant virus particles comprise a plant virus from the family Tymoviridae.