US 11,946,059 B2
In vivo generation of water-soluble cannabinoids in plant cell suspension cultures
Richard T. Sayre, Los Alamos, NM (US); Elton Carvalho Gonçalves, Los Alamos, NM (US); Tawanda Zidenga, White Rock, NM (US); and Stephanie Willette, Santa Fe, NM (US)
Assigned to TRAIT BIOSCIENCES, INC., Los Alamos, NM (US)
Filed by Trait Biosciences Inc., Los Alamos, NM (US)
Filed on May 30, 2019, as Appl. No. 16/426,059.
Application 16/426,059 is a continuation in part of application No. 16/110,954, filed on Aug. 23, 2018.
Application 16/110,954 is a continuation in part of application No. PCT/US2018/041710, filed on Jul. 11, 2018.
Application PCT/US2018/041710 is a continuation in part of application No. PCT/US2018/024409, filed on Mar. 26, 2018.
Claims priority of provisional application 62/531,123, filed on Jul. 11, 2017.
Claims priority of provisional application 62/588,662, filed on Nov. 20, 2017.
Claims priority of provisional application 62/621,166, filed on Jan. 24, 2018.
Prior Publication US 2019/0316144 A1, Oct. 17, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); A61K 31/352 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01)
CPC C12N 15/8257 (2013.01) [A61K 31/352 (2013.01); C12N 9/0042 (2013.01); C12N 9/1051 (2013.01); C12N 15/8201 (2013.01); C12Y 106/02004 (2013.01); C12Y 204/01 (2013.01)] 1 Claim
 
1. A method of glycosylating a cannabinoid, comprising the steps of:
(a) establishing a tobacco cell suspension culture of Nicotiana tabacum or Nicotiana benthamiana cells;
(b) introducing into said tobacco cell suspension culture a cannabinoid, wherein the cannabinoid is cannabidiol (CBD) or cannabidiolic acid (CBDA);
(c) incubating said cannabinoid in said tobacco cell suspension culture, thereby glycosylating the cannabinoid;
(d) isolating a cannabinoid glycoside from said tobacco cell suspension culture, wherein the cannabinoid glycoside is a CBD-glycoside or a CBDA-glycoside.