US 11,946,044 B2
Methods for isolating cell-free DNA
Ariel Jaimovich, Redwood City, CA (US); Sven Jeffrey Duenwald, Millbrae, CA (US); Peter Vilem Grauman, Philadelphia, PA (US); Yupeng He, Redwood City, CA (US); Charbel Said Eid, Millbrae, CA (US); and Haley Dara Axelrod, Los Altos, CA (US)
Assigned to Guardant Health, Inc., Palo Alto, CA (US)
Filed by GUARDANT HEALTH, INC., Redwood City, CA (US)
Filed on Jul. 29, 2021, as Appl. No. 17/389,199.
Claims priority of provisional application 63/059,029, filed on Jul. 30, 2020.
Prior Publication US 2022/0195419 A1, Jun. 23, 2022
Int. Cl. C12N 15/10 (2006.01); C12Q 1/6886 (2018.01)
CPC C12N 15/1065 (2013.01) [C12Q 1/6886 (2013.01); C12Q 2600/154 (2013.01); C12Q 2600/156 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method of isolating DNA from a sample, the method comprising:
partitioning the DNA of the sample into a plurality of partitions, the plurality of partitions comprising at least a hypermethylated partition and a hypomethylated partition;
preparing a first pool comprising at least a first portion of the DNA from the hypomethylated partition;
preparing a second pool comprising at least a first portion of the DNA from the hypermethylated partition;
capturing at least a first set of target regions from the first pool; and
capturing at least a second set of target regions from the second pool,
wherein the first set of target regions and the second set of target regions are not identical, and further wherein:
(a) the first pool comprises only a portion of the DNA of the hypermethylated partition;
and/or
(b) the plurality of partitions further comprises an intermediately methylated partition and the second pool comprises only a portion of the DNA of the intermediately methylated partition.