	US 11,946,037 B2
	Method for digesting nucleic acid in a sample
Justin Joseph O'Grady, Norwich (GB); Gemma Louise Kay, Norwich (GB); Themoula Charalampous, Norwich (GB); Alp Aydin, Norwich (GB); and Riccardo Scotti, Norwich (GB)
Assigned to UEA Enterprises Limited, Norwich (GB)
Appl. No. 17/779,414
Filed by UEA Enterprises Limited, Norwich (GB)
PCT Filed Nov. 24, 2020, PCT No. PCT/GB2020/052986 § 371(c)(1), (2) Date May 24, 2022, PCT Pub. No. WO2021/105659, PCT Pub. Date Jun. 3, 2021.
Claims priority of application No. 1917101 (GB), filed on Nov. 25, 2019.
Prior Publication US 2023/0203472 A1, Jun. 29, 2023
Int. Cl. C12N 15/10 (2006.01); C12N 9/22 (2006.01)

CPC C12N 15/1003 (2013.01) [C12N 9/22 (2013.01)]

13 Claims

1. A method for depleting host nucleic acid in a biological sample, said biological sample having been previously obtained from an animal host, wherein said biological sample comprises animal host cells, comprising:

a) adding to said biological sample a saponin, a DNase, and NaCl and/or KCl, to form a reaction mix, wherein the addition of said NaCl and/or KCl is sufficient to ensure a final concentration of NaCl and/or KCl in the reaction mix of at least 0.2M; and

b) incubating the reaction mix at between 10° C. and 50° C.;

wherein, in said method, lysis of host cells and DNase degradation of host nucleic acid occur at least partially in parallel.