US 11,946,035 B2
Microfluidic-enabled multiwell cell culture devices and systems for precision culture, control and monitoring of living cells
Mary J. C. Ludlam, San Francisco, CA (US); Austin Blanco, Lincoln, CA (US); David Wartmann, Berkeley, CA (US); and Richard A. Mathies, Walnut Creek, CA (US)
Assigned to The Regents of The University of California, Oakland, CA (US); and Cairn Biosicences, Inc., San Francisco, CA (US)
Appl. No. 16/614,290
Filed by Cairn Biosciences, Inc., San Francisco, CA (US); and The Regents of the University of California, Oakland, CA (US)
PCT Filed May 15, 2018, PCT No. PCT/US2018/032838
§ 371(c)(1), (2) Date Nov. 15, 2019,
PCT Pub. No. WO2018/213357, PCT Pub. Date Nov. 22, 2018.
Claims priority of provisional application 62/507,139, filed on May 16, 2017.
Prior Publication US 2021/0002602 A1, Jan. 7, 2021
Int. Cl. C12M 3/06 (2006.01); C12M 1/00 (2006.01); C12M 1/32 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); G06F 3/0481 (2022.01)
CPC C12M 41/48 (2013.01) [C12M 23/12 (2013.01); C12M 23/16 (2013.01); C12M 29/00 (2013.01); C12M 29/20 (2013.01); C12M 41/44 (2013.01); G06F 3/0481 (2013.01)] 47 Claims
OG exemplary drawing
 
1. A microfluidic-enabled multiwell device for microfluidic control of fluids for cell cultures comprising:
a microfluidics module comprising a well layer, a fluid channels layer, and a pneumatic layer, wherein the well layer is configured to be removable from the microfluidics module;
a sensor module comprising one or more sensors configured to detect data regarding an environment inside the microfluidic module;
one or more processors; and
memory storing instructions configured to be executed by the one or more processors to cause the multiwell device to execute a cell culture process, comprising:
monitoring, by the sensor module, one or more parameters of an external environment surrounding the multiwell device;
in accordance with monitoring the one or more external environmental parameters, adjusting one or more parameters of the environment inside the microfluidics module;
receiving data collected from the one or more sensors regarding the environment inside the microfluidic module; and
based at least in part on the data received, causing fluid to flow to an individually addressable well in the multiwell device.