US 11,945,873 B2
Antitumor antagonists
Jackie Sheng, Thousand Oaks, CA (US); Bo Liu, Thousand Oaks, CA (US); and Margaret Karow, Santa Rosa Valley, CA (US)
Assigned to GENSUN BIOPHARMA, INC., Newbury Park, CA (US)
Filed by GENSUN BIOPHARMA, INC., Newbury Park, CA (US)
Filed on Apr. 16, 2021, as Appl. No. 17/301,869.
Application 17/301,869 is a continuation of application No. 16/457,399, filed on Jun. 28, 2019, granted, now 11,001,635.
Claims priority of provisional application 62/823,989, filed on Mar. 26, 2019.
Claims priority of provisional application 62/691,658, filed on Jun. 29, 2018.
Prior Publication US 2021/0246214 A1, Aug. 12, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/71 (2006.01); A61P 35/00 (2006.01); C07K 14/00 (2006.01); C07K 16/22 (2006.01); C07K 16/28 (2006.01); C07K 16/46 (2006.01); A61K 39/00 (2006.01)
CPC C07K 16/2863 (2013.01) [A61P 35/00 (2018.01); C07K 14/00 (2013.01); C07K 14/71 (2013.01); C07K 16/22 (2013.01); C07K 16/2803 (2013.01); C07K 16/2818 (2013.01); C07K 16/2827 (2013.01); C07K 16/468 (2013.01); A61K 2039/505 (2013.01); C07K 2317/31 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01); C07K 2317/64 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01); C07K 2318/10 (2013.01); C07K 2319/30 (2013.01); C07K 2319/32 (2013.01)] 6 Claims
 
1. A bispecific antibody comprising two heavy chains and two light chains, wherein each heavy chain comprises, from N-terminus to C-terminus, a VH domain, a CHI domain, a CH2 domain, a CH3 domain, a peptide linker and a TGF-ß1 RII extracellular domain (ECD), wherein each light chain comprises, from N-terminus to C-terminus, a VL domain and a CL domain, wherein the VH domain comprises the amino acid sequence of SEQ ID NO:90 or 96, wherein the VL domain comprises the amino acid sequence of SEQ ID NO:91, wherein the bispecific antibody binds specifically to (1) human VEGF-A through the VH and VL domains and (2) human TGFß1 through the TGF-ß1 RII extracellular domain (ECD), wherein the TGF-ß1 R11 ECD comprises the amino acid sequence of SEQ ID NO:89.