US 11,945,234 B2
Laser-induced cell transfer and sorting
Jun Zhang, Munich (DE); Heinz Paul Huber, Oberhaching (DE); Hauke Clausen-Schaumann, Bernried (DE); Stefanie Sudhop, Fahrenzhauen (DE); and Denitsa Docheva, Regensburg (DE)
Assigned to Hochschule für angewandte Wissenschaften München, Munich (DE); and Universität Regensburg—Universitätsklinikum, Regensburg (DE)
Appl. No. 17/413,189
Filed by Hochschule für angewandte Wissenschaften München, Munich (DE); and Universität Regensburg—Universitätsklinikum, Regensburg (DE)
PCT Filed Dec. 19, 2019, PCT No. PCT/EP2019/086173
§ 371(c)(1), (2) Date Jun. 11, 2021,
PCT Pub. No. WO2020/127640, PCT Pub. Date Jun. 25, 2020.
Claims priority of application No. 18214358 (EP), filed on Dec. 20, 2018.
Prior Publication US 2021/0339539 A1, Nov. 4, 2021
Int. Cl. B41J 2/44 (2006.01); C12M 1/26 (2006.01); C12Q 1/24 (2006.01)
CPC B41J 2/44 (2013.01) [C12M 33/00 (2013.01); C12Q 1/24 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for transferring objects onto a substrate, the method comprising:
providing a medium in a reservoir, wherein the medium contains objects;
providing the substrate having an acceptor surface, wherein the acceptor surface faces an opening of the reservoir;
determining a first target focus point in the medium; and
generating a first laser pulse or first laser pulse train focused onto the first target focus point,
wherein:
one or both of a pulse intensity of the first laser pulse or first laser pulse train at the first target focus point and a pulse duration of the first laser pulse or first laser pulse train is/are chosen such that the first laser pulse or first laser pulse train generates a droplet of the medium that is ejected from the medium towards the acceptor surface;
a center wavelength of the first laser pulse or first laser pulse train is larger than 500 nm;
a position of at least one of the objects in the medium is determined at least in part by determining at least two spatial coordinates of the position of the at least one object; and
the first target focus point is determined relative to the position of the at least one object.