US 11,944,720 B2
Method and device for preparing an implant obtained from a culture of stem cells
Karim Ben M'Barek, Creteil (FR); Walter Habeler, Paris (FR); and Christelle Monville, Champigny sur Marne (FR)
Assigned to CENTRE D'ETUDE DES CELLULES SOUCHES (CECS), Corbeil-Essonnes (FR)
Appl. No. 16/980,120
Filed by CENTRE D'ETUDE DES CELLULES SOUCHES, Corbeil-Essonnes (FR)
PCT Filed Mar. 12, 2019, PCT No. PCT/FR2019/050529
§ 371(c)(1), (2) Date Sep. 11, 2020,
PCT Pub. No. WO2019/175497, PCT Pub. Date Sep. 19, 2019.
Claims priority of application No. 18/52114 (FR), filed on Mar. 12, 2018.
Prior Publication US 2021/0023273 A1, Jan. 28, 2021
Int. Cl. A61L 27/38 (2006.01); A61L 27/52 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 3/00 (2006.01); C12N 5/071 (2010.01); C12N 5/0735 (2010.01); C12N 5/079 (2010.01)
CPC A61L 27/3834 (2013.01) [A61L 27/3813 (2013.01); A61L 27/3869 (2013.01); A61L 27/52 (2013.01); C12M 21/08 (2013.01); C12M 23/06 (2013.01); C12M 23/38 (2013.01); C12M 25/04 (2013.01); C12N 5/0606 (2013.01); C12N 5/0621 (2013.01); C12N 5/069 (2013.01); C12N 5/0697 (2013.01); A61L 2400/06 (2013.01); A61L 2430/16 (2013.01); C12N 2502/02 (2013.01); C12N 2502/085 (2013.01); C12N 2502/28 (2013.01)] 20 Claims
 
1. A method of preparing an implant (70) comprising a culture of cells (73) on a membrane (34), the method comprising the steps of: fixing the membrane on a support (30), disposing the support in a housing recess (16) of a housing (10, 20) leaving two spaces (38, 39) having adjusted heights, above and below the membrane, injecting into the spaces a liquid capable of transforming into gel at a temperature of transport below an injection temperature of the liquid, and bringing the housing to the transport temperature, so as to form an implant comprising the membrane and two layers of gel (71, 72) having adjusted thicknesses, respectively, on two opposite faces of the membrane.