	US 11,944,672 B2
	Therapeutic vaccine for treatment of diabetes type 1 in children, application of the cell sorter and the method of multiplying Treg cells to produce therapeutic vaccine for treatment of diabetes type 1
Piotr Trzonkowski, Sopot (PL); Malgorzata Mysliwiec, Gdansk (PL); and Natalia Marek-Trzonkowska, Gdansk (PL)
Assigned to Gdanski Uniwersytet Medyczny, Gdansk (PL)
Filed by Gdanski Uniwersytet Medyczny, Gdansk (PL)
Filed on Dec. 22, 2017, as Appl. No. 15/852,227.
Application 15/852,227 is a continuation of application No. 14/405,906, abandoned, previously published as PCT/PL2013/000072, filed on Jun. 4, 2013.
Claims priority of application No. 399447 (PL), filed on Jun. 6, 2012.
Prior Publication US 2018/0117134 A1, May 3, 2018
Int. Cl. A61K 39/00 (2006.01); A61K 35/17 (2015.01); A61K 35/26 (2015.01); C12N 5/0783 (2010.01); C12N 13/00 (2006.01)

CPC A61K 39/0008 (2013.01) [A61K 35/17 (2013.01); A61K 35/26 (2013.01); C12N 5/0637 (2013.01); C12N 13/00 (2013.01); A61K 2039/5158 (2013.01); A61K 2039/55 (2013.01); C12N 2501/2302 (2013.01); C12N 2502/11 (2013.01)]

14 Claims

1. A method of treating a child with type 1 diabetes comprising:

administering to the child CD3(+)CD4(+)CD25(high)CD127(−) autologous Treg cells expanded ex vivo from the child in an amount between 10×10⁶cells/kg body weight and 30×10⁶cells/kg body weight,

wherein more than 90% of the Treg cells express factor FoxP3, and

wherein the administration of the Treg cells results in an increase in the C-peptide level in the child at 5 months after the administration.