US 11,944,644 B2
T-cells comprising anti-CD38 and anti-CD138 chimeric antigen receptors and uses thereof
Zelig Eshhar, Tel Yitzhak (IL); Tova Waks, Petach Tikva (IL); Anat Globerson Levin, Tel Aviv (IL); and Moran Rawet Slobodkin, Tel Aviv (IL)
Assigned to THE MEDICAL RESEARCH INFRASTRUCTURE AND HEALTH SERVICES FUND OF THE TEL AVIV MEDICAL CENTER, Tel Aviv (IL); and YEDA RESEARCH AND DEVELOPMENT CO. LTD, Rehovot (IL)
Appl. No. 16/769,111
Filed by THE MEDICAL RESEARCH INFRASTRUCTURE AND HEALTH SERVICES FUND OF THE TEL AVIV MEDICAL CENTER, Tel Aviv (IL); and YEDA RESEARCH AND DEVELOPMENT CO. LTD., Rehovot (IL)
PCT Filed Dec. 4, 2018, PCT No. PCT/IL2018/051325
§ 371(c)(1), (2) Date Jun. 2, 2020,
PCT Pub. No. WO2019/111249, PCT Pub. Date Jun. 13, 2019.
Claims priority of provisional application 62/667,638, filed on May 7, 2018.
Claims priority of provisional application 62/594,577, filed on Dec. 5, 2017.
Prior Publication US 2020/0384024 A1, Dec. 10, 2020
Int. Cl. A61K 39/00 (2006.01); A61K 35/17 (2015.01); A61K 39/395 (2006.01); A61P 35/00 (2006.01); C07K 16/28 (2006.01); C07K 16/30 (2006.01); C12N 5/0783 (2010.01); C12N 5/10 (2006.01); C12N 15/63 (2006.01)
CPC A61K 35/17 (2013.01) [A61K 39/001126 (2018.08); A61K 39/001129 (2018.08); A61K 39/3955 (2013.01); A61K 39/39558 (2013.01); A61K 39/4631 (2023.05); A61P 35/00 (2018.01); C07K 16/28 (2013.01); C07K 16/2896 (2013.01); C07K 16/3061 (2013.01); C12N 5/0636 (2013.01); C12N 5/10 (2013.01); C12N 15/63 (2013.01); A61K 2039/505 (2013.01); C07K 16/30 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/56 (2013.01); C07K 2317/569 (2013.01); C07K 2317/76 (2013.01); C07K 2319/03 (2013.01)] 19 Claims
 
1. A T-cell genetically modified to express at least two distinct separate chimeric antigen receptors (CARs), wherein the first CAR comprises an antigen binding domain that binds specifically to CD138 and the second CAR comprises an antigen binding domain that binds specifically to CD38, wherein one of the CARs comprises an activation domain and is devoid of a costimulatory domain and the other CAR comprises a costimulatory domain and is devoid of an activation domain.