US 11,944,435 B2
System and procedure for stabilizing, storing and recovering blood samples
Randy Ringold, West Hills, CA (US); Kyle Wilson, Simi Valley, CA (US); Tyson Ringold, Toronto (CA); and Ekaterina Pesherov, Encino, CA (US)
Appl. No. 16/622,040
Filed by VDI Laboratory, LLC, Simi Valley, CA (US)
PCT Filed Jun. 13, 2018, PCT No. PCT/US2018/037302
§ 371(c)(1), (2) Date Dec. 12, 2019,
PCT Pub. No. WO2018/231960, PCT Pub. Date Dec. 20, 2018.
Claims priority of provisional application 62/519,171, filed on Jun. 13, 2017.
Prior Publication US 2020/0170557 A1, Jun. 4, 2020
Int. Cl. A61B 5/15 (2006.01); A61B 10/00 (2006.01); B01L 3/00 (2006.01); G01N 1/30 (2006.01)
CPC A61B 5/150358 (2013.01) [A61B 5/150305 (2013.01); A61B 10/0096 (2013.01); B01L 3/5021 (2013.01); B01L 3/505 (2013.01); G01N 1/30 (2013.01); A61B 2010/0006 (2013.01); B01L 2200/18 (2013.01); B01L 2300/0618 (2013.01); B01L 2300/0809 (2013.01); B01L 2300/105 (2013.01); B01L 2300/123 (2013.01); B01L 2300/126 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method for stabilizing analytes in blood samples, and for delayed extraction of blood samples from dried blood spots resulting in near neat-level blood samples for use in tests in human and veterinary applications, said method comprising steps of:
obtaining a blood sample from a subject, placing said blood sample in a device for holding said blood sample;
placing a test tube elevator in a sealable centrifuge tube and a buffer solution in a sealable centrifuge tube, wherein said test tube elevator includes a tubular portion vertically extended along a height of said sealable centrifuge tube and a planar portion perpendicular to said tubular portion and radially extended from said tubular portion toward an inner surface of said sealable centrifuge tube, wherein said planar portion is configured to divide said sealable centrifuge tube into an upper portion and a lower portion, and wherein said planar portion includes a plurality of holes for passing liquid between said lower and upper portions;
obtaining at least one strip of absorbent paper containing said blood sample from a dried blood holding device and placing said at least one strip of absorbent paper in said sealable centrifuge tube on top of said test tube elevator, wherein said at least one strip of absorbent paper is not in contact with a bottom of said sealable centrifuge tube;
incubating said at least one strip of absorbent paper in said buffer solution inside by holding said sealable centrifuge tube flipped upside-down; and
centrifuging said sealable tube while directing a centrifuge force toward a bottom of said sealable centrifuge tube to push said buffer solution toward the bottom of said sealable centrifuge tube, while said at least one strip of absorbent paper remains held by said test tube elevator close to a top of said sealable centrifuge tube.