| CPC G01N 33/689 (2013.01) [C12Q 1/6844 (2013.01); G01N 21/645 (2013.01); G01N 21/6486 (2013.01); G01N 2021/6482 (2013.01)] | 18 Claims |
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1. A method, comprising:
(a) distributing a sample comprising a set of nucleic acids, across a set of partitions comprising at least 20 million partitions;
(b) amplifying the set of nucleic acids within the set of partitions;
(c) in a container, detecting signals corresponding to target nucleic acids of the set of nucleic acids, from multiple partitions of the set of partitions simultaneously, upon scanning a cross-section of the container; and
(d) counting partitions comprising signals detected in (c), wherein the counting does not comprise use of Poisson statistics associated correction factors wherein the set of partitions comprises a set of droplets, wherein the set of droplets are of an emulsion, wherein a given droplet of the set of droplets of the emulsion: (i) is in a continuous phase of the emulsion; and (ii) is surrounded by an immiscible film, wherein the immiscible film is immiscible with the continuous phase and with the given droplet of the set of droplets.
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