	US 12,265,083 B2
	Immunoassay method for free AIM in biological sample, and assay kit
Toru Miyazaki, Tokyo (JP); Takeshi Okanoue, Suita (JP); Tomohide Asai, Tokyo (JP); Yuka Kanetsuki, Tokyo (JP); and Jiro Hirota, Tokyo (JP)
Assigned to SEKISUI MEDICAL CO., LTD., Tokyo (JP); Miyazaki Toru, Tokyo (JP); and SOCIAL WELFARE ORGANIZATION “SAISEIKAI” IMPERIAL GIFT FOUNDATION INC., Tokyo (JP)
Appl. No. 17/427,559
Filed by SEKISUI MEDICAL CO., LTD., Tokyo (JP); Toru Miyazaki, Tokyo (JP); and SOCIAL WELFARE ORGANIZATION “SAISEIKAI” IMPERIAL GIFT FOUNDATION INC., Tokyo (JP)
PCT Filed Jan. 30, 2020, PCT No. PCT/JP2020/003415 § 371(c)(1), (2) Date Jul. 30, 2021, PCT Pub. No. WO2020/158857, PCT Pub. Date Aug. 6, 2020.
Claims priority of application No. 2019-015220 (JP), filed on Jan. 31, 2019.
Prior Publication US 2022/0107318 A1, Apr. 7, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/53 (2006.01); G01N 33/577 (2006.01)

CPC G01N 33/577 (2013.01) [G01N 33/53 (2013.01); G01N 33/5306 (2013.01)]

7 Claims

1. An immunoassay method for measuring an amount of free apoptosis inhibitor of macrophage (AIM) in a biological sample containing complex AIM and free AIM, the method comprising: bringing the biological sample into contact with an anti-AIM antibody in a presence of an added anti-IgM antibody at a concentration sufficient to reduce a non-specific reaction.