US 12,265,073 B2
Method and system for chromogenic array-based food testing
Boce Zhang, Dracut, MA (US); Hengyong Yu, Westford, MA (US); Yaguang Luo, Bethesda, MD (US); and Xiaobo Liu, Dracut, MA (US)
Assigned to University of Massachusetts, Boston, MA (US)
Appl. No. 17/292,133
Filed by University of Massachusetts, Boston, MA (US); and The United States of America, as Represented by the Secretary of Agriculture, Washington, DC (US)
PCT Filed Nov. 5, 2019, PCT No. PCT/US2019/059816
§ 371(c)(1), (2) Date May 7, 2021,
PCT Pub. No. WO2020/097043, PCT Pub. Date May 14, 2020.
Claims priority of provisional application 62/757,388, filed on Nov. 8, 2018.
Prior Publication US 2021/0396730 A1, Dec. 23, 2021
Int. Cl. G01N 33/00 (2006.01); G01N 31/22 (2006.01); G01N 33/12 (2006.01); G01N 33/14 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/12 (2013.01) [G01N 31/22 (2013.01); G01N 33/0047 (2013.01); G01N 33/14 (2013.01); G01N 33/583 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A chromogenic assay, comprising
a substrate comprising an array of 10 or more dyes which react with volatile organic compounds, wherein each of the dyes of the array of 10 or more dyes are chromogenic when reacted with volatile organic chemical (VOC) biomarkers, wherein the VOC biomarkers comprise acids, alcohols, aldehydes, alkenes, amines, antioxidants, aromatic compounds, esters, ethylene, lactones, ketones, organosulfur compounds, sulfides, reactive oxygen species, terpenes, or a combination thereof; wherein the array of 10 or more dyes comprises 2,4-dinitrophenylhydrazine (phenylhydrazine), 4,4′-azodianiline (dianiline) and pararosaniline (fuchsine), bromophenol blue, nitrazine yellow, chlorophenol red, Tollen's reagent, Benedict's reagent, zinc nitrate, and sodium nitroprusside.