US 12,265,033 B2
Method, computer program and apparatus for determining positions of molecules in a sample
Joachim Fischer, Karlsruhe (DE); Matthias Henrich, Heidelberg (DE); and Winfried Willemer, Bovenden (DE)
Assigned to ABBERIOR INSTRUMENTS GMBH, Gottingen (DE)
Filed by ABBERIOR INSTRUMENTS GMBH, Gottingen (DE)
Filed on Nov. 21, 2022, as Appl. No. 17/991,447.
Application 17/991,447 is a continuation in part of application No. PCT/EP2021/063788, filed on May 25, 2021.
Claims priority of application No. 10 2020 113 998.5 (DE), filed on May 26, 2020.
Prior Publication US 2023/0101017 A1, Mar. 30, 2023
Int. Cl. G01N 21/64 (2006.01); G02B 21/36 (2006.01)
CPC G01N 21/6458 (2013.01) [G01N 21/6428 (2013.01); G02B 21/361 (2013.01); G02B 21/365 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A method for determining positions of two or more spaced-apart molecules in one or more spatial directions in a sample using a localization microscope, wherein light distributions arising due to interference of coherent light are used for determining the positions of the molecules, comprising:
generating a plurality of light distributions using a first light modulator having a plurality of switchable pixels disposed in an image plane of the localization microscope, each light distribution having at least one local intensity minimum and regions with an intensity increase adjacent thereto;
illuminating each of the two or more molecules with one light distribution each;
for each of the light distributions, detecting photons emitted by the molecules for different positionings of the light distribution, the light distributions being positioned independently of each other; and
deriving the positions of the molecules based on the photons detected for the different positionings of the light distributions.