US 12,265,030 B2
Hybrid multi-photon microscopy
Alipasha Vaziri, New York, NY (US); and Siegfried Weisenburger, New York, NY (US)
Assigned to The Rockefeller University, New York, NY (US)
Filed by The Rockefeller University, New York, NY (US)
Filed on Oct. 2, 2023, as Appl. No. 18/479,321.
Application 18/479,321 is a continuation of application No. 17/427,586, granted, now 11,808,702, previously published as PCT/US2020/015825, filed on Jan. 30, 2020.
Claims priority of provisional application 62/799,408, filed on Jan. 31, 2019.
Prior Publication US 2024/0159674 A1, May 16, 2024
Int. Cl. G01N 21/64 (2006.01); G02B 21/16 (2006.01)
CPC G01N 21/6428 (2013.01) [G01N 21/6458 (2013.01); G02B 21/16 (2013.01); G01N 2201/06113 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A multi-photon imaging system comprising:
a laser module including a pulse amplifier, the pulse amplifier outputting a two-photon excitation laser pulse and a three-photon excitation laser pulse, the laser module having a first output channel for guiding the two-photon excitation laser pulse in a first optical path and a second output channel for guiding the three-photon excitation laser pulse in a second optical path;
a telescope for receiving the two-photon excitation laser pulse from the first optical path and the three-photon excitation laser pulse from the second optical path, and delivering the two-photon excitation laser pulse and the three-photon excitation pulse to a target volume; and
a detector configured to collect photons generated within the target volume in response to excitation of the target volume by both the two-photon excitation laser pulse and the three-photon excitation laser pulse.