US 12,265,015 B2
Method for optimizing microparticle suction conditions, microparticle sorting device, microparticle sorting system, and microparticle sorting program
Gakuji Hashimoto, Kanagawa (JP)
Assigned to Sony Corporation, Tokyo (JP)
Appl. No. 16/976,060
Filed by Sony Corporation, Tokyo (JP)
PCT Filed Jan. 28, 2019, PCT No. PCT/JP2019/002659
§ 371(c)(1), (2) Date Aug. 26, 2020,
PCT Pub. No. WO2019/167510, PCT Pub. Date Sep. 6, 2019.
Claims priority of application No. 2018-037468 (JP), filed on Mar. 2, 2018.
Prior Publication US 2021/0372917 A1, Dec. 2, 2021
Int. Cl. G01N 15/14 (2024.01); G01N 15/10 (2024.01); G01N 15/1404 (2024.01); G01N 15/149 (2024.01)
CPC G01N 15/1484 (2013.01) [G01N 15/1404 (2013.01); G01N 15/1459 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1486 (2013.01); G01N 15/149 (2024.01)] 3 Claims
OG exemplary drawing
 
1. A method for optimizing microparticle suction conditions, the method using a microparticle sorting microchip including: a main flow channel in which a sheath solution and a microparticle-containing sample solution flow; and a pressure chamber that sucks microparticles,
the method comprising:
a step of acquiring data of a velocity V of each adjustment microparticle, by introducing the sheath solution and an adjustment-microparticle-containing sample solution into the main flow channel, and detecting a point of time at which the adjustment microparticle passes through a predetermined position in the main flow channel;
a step of sucking the adjustment microparticles from the main flow channel into the pressure chamber with a predetermined suction force;
a step of counting the number of the adjustment microparticles sucked into the pressure chamber;
a step of determining a time elapsed since passage through the predetermined position, on a basis of a time from the point of time at which the adjustment microparticle passed through the predetermined position in the main flow channel till the suctioning is performed, and the number of the counted adjustment microparticles, the suctioning being to be performed after the elapsed time;
a step of acquiring data of a velocity V of each adjustment microparticle, and an average velocity Vave during a certain time, by introducing a larger amount of sample solution than the adjustment-microparticle-containing sample solution into the main flow channel, and detecting a point of time at which the adjustment microparticle passes through the predetermined position in the main flow channel; and
a step of sorting the adjustment microparticles into the pressure chamber, by controlling the pressure in the pressure chamber sucking the adjustment microparticles, in inverse proportion to an index calculated by V/Vave.