US 12,265,014 B2
Systems and methods for evaluating immune response to infection via monitoring cell granularity parameter of cells
Liliana Tejidor, Coral Gables, FL (US); Robert T. Magari, Cooper City, FL (US); Diana Careaga, Miami, FL (US); and Sanghyuk Shin, Carlsbad, CA (US)
Assigned to Beckman Coulter, Inc., Brea, CA (US)
Filed by Beckman Coulter, Inc., Brea, CA (US)
Filed on Sep. 13, 2023, as Appl. No. 18/367,705.
Application 18/367,705 is a division of application No. 16/925,937, filed on Jul. 10, 2020, granted, now 11,796,447.
Claims priority of provisional application 62/873,575, filed on Jul. 12, 2019.
Prior Publication US 2024/0053253 A1, Feb. 15, 2024
Int. Cl. G01N 15/14 (2024.01); G01N 33/80 (2006.01); G01N 15/01 (2024.01); G01N 15/10 (2006.01); G01N 33/48 (2006.01)
CPC G01N 15/1459 (2013.01) [G01N 33/80 (2013.01); G01N 2015/012 (2024.01); G01N 2015/1006 (2013.01); G01N 33/48 (2013.01); G01N 2800/26 (2013.01); G01N 2800/52 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A system for characterizing an inflammatory response to infection, the system comprising:
a. a transducer module configured to measure at least a cell granularity parameter of cells passing through a flowcell; and
b. a processor configured with instructions stored on a non-transitory computer readable medium and operable to, when executed and based on measurement data from the transducer module, detect whether a differentially expressed sepsis cell population parameter is present in a heterogenous population of circulating cells passing through the flowcell by performing steps comprising:
i. identifying one or more cells among the heterogenous population of circulating cells passing through the flowcell;
ii. obtaining a plurality of measurements, wherein the plurality of measurements comprises, for the one or more cells a measurement of the cell granularity parameter; and
iii. calculating a standard deviation of the measurements of the cell granularity parameter for the one or more cells.