US 12,265,003 B2
Inkjet deposition of reagents for histological samples
Henning Groll, Tucson, AZ (US); Melis Hazar, Tucson, AZ (US); Eric L. Klein, Tucson, AZ (US); Raymond T. Kozikowski, III, Tucson, AZ (US); Chana R. Paluszcyk, Tucson, AZ (US); Melinda J. Polaske, Oracle, AZ (US); and Raviraj Thakur, West Lafayette, IN (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Dec. 8, 2022, as Appl. No. 18/077,302.
Application 18/077,302 is a division of application No. 15/782,816, filed on Oct. 12, 2017, granted, now 11,561,156.
Application 15/782,816 is a continuation of application No. PCT/EP2016/058801, filed on Apr. 20, 2016.
Claims priority of provisional application 62/150,122, filed on Apr. 20, 2015.
Prior Publication US 2023/0105136 A1, Apr. 6, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 1/31 (2006.01); G01N 33/53 (2006.01); G01N 35/10 (2006.01); G01N 1/30 (2006.01)
CPC G01N 1/312 (2013.01) [G01N 33/53 (2013.01); G01N 35/1002 (2013.01); G01N 35/1011 (2013.01); G01N 35/1016 (2013.01); G01N 1/30 (2013.01); G01N 2001/302 (2013.01); G01N 2001/317 (2013.01); G01N 2035/1041 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method of staining a biological sample disposed on a microscope slide with a primary stain reagent, comprising:
identifying one or more regions of the biological sample for receiving the primary stain reagent;
determining a dispense pattern based on the one or more identified regions; and
dispensing, based on the determined dispense pattern, between about 360 nL/in2 to about 14.4 μL/in2 of the primary stain reagent per pass to the biological sample with a piezoelectric deposition system or a thermal droplet generation system;
wherein the primary stain reagent comprises a dye.