US 12,264,369 B2
Real-time PCR for the detection of pathogens
Bernard J. Wolff, Roswell, GA (US); Jonas M. Winchell, Lilburn, GA (US); and Maureen Diaz, Atlanta, GA (US)
Assigned to The Government of the United States of America as represented by the Secretary of the Deptartment of Health and Human Services, Bethesda, MD (US)
Filed by The Government of the USA as represented by the Secretary of the Dept. of Health and Human Services, Atlanta, GA (US)
Filed on Sep. 27, 2021, as Appl. No. 17/485,953.
Application 17/485,953 is a division of application No. 16/056,147, filed on Aug. 6, 2018, granted, now 11,162,144.
Application 16/056,147 is a division of application No. 14/398,390, granted, now 10,072,305, issued on Sep. 11, 2018, previously published as PCT/US2013/028034, filed on Feb. 27, 2013.
Claims priority of provisional application 61/642,091, filed on May 3, 2012.
Prior Publication US 2022/0010365 A1, Jan. 13, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/689 (2018.01); C12Q 1/6895 (2018.01)
CPC C12Q 1/689 (2013.01) [C12Q 1/6895 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01)] 23 Claims
 
1. A method for detecting presence of Klebsiella pneumoniae in a sample, comprising:
contacting the sample with a nucleic acid probe comprising the nucleic acid sequence of SEQ ID NO: 60, or the reverse complement thereof, and a donor fluorophore, an acceptor fluorophore, or a combination thereof;
amplifying a nucleic acid from Klebsiella pneumoniae, wherein the amplifying comprises contacting the sample with a pair of primers comprising the nucleic acid sequences of SEQ ID NOs: 58 and 59; and
detecting hybridization between the nucleic acid probe and a nucleic acid in the sample, wherein detection of hybridization indicates the presence of Klebsiella pneumoniae in the sample.