| CPC C12Q 1/6874 (2013.01) [B01J 19/0046 (2013.01); C12N 15/1065 (2013.01); C40B 20/04 (2013.01); C40B 70/00 (2013.01); B01J 2219/00547 (2013.01); B01J 2219/00572 (2013.01); B01J 2219/00722 (2013.01)] | 20 Claims |
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1. A system for nucleic acid sequencing, comprising:
a plurality of reagent sources respectively containing differing species of nucleotides;
a reaction chamber array comprising a plurality of reaction sites each containing a tagged polynucleotide comprising a sample polynucleotide and a combinatorial barcode sequence, wherein:
the combinatorial barcode sequence comprises a plurality of iterations of a sequence motif,
the sequence motif comprises a first nucleotide base from a first group of nucleotide bases followed by a second nucleotide base from a second group of nucleotide bases, the first group of nucleotide bases and the second group of nucleotide bases differing from each other, and
the combinatorial barcode sequence being one of at least eight potential combinatorial barcode sequences, the potential combinatorial barcode sequences exhibiting synchronization in flow space in response to being sequenced based on a predetermined order of nucleotide flows, the predetermined order of nucleotide flows comprising a repetition of a flow order motif complementary to the sequence motif,
a flow control system configured to control respective flows from the plurality of reagent sources to the plurality of reaction sites of the reaction chamber array so as to expose the tagged polynucleotide at a respective reaction site to sequential nucleotide flows, each flow comprising one species of nucleotide and the sequential nucleotide flows being in a predetermined order based on the species of nucleotide;
a detection component configured to detect a series of signals over the combinatorial barcode sequence resulting from incorporation of nucleotides from the sequential nucleotide flows into one or more of the tagged polynucleotides; and
a computing device comprising a processor configured to resolve the detected series of signals to determine the combinatorial barcode sequence.
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