	US 12,264,352 B2
	Method for enhancing extracellular vesicle production
Hiroshi Matsui, Glen Rock, NJ (US); Kiyotaka Shiba, Tokyo (JP); and Min A. Kang, Flushing, NY (US)
Assigned to Research Foundation of the City University of New York, New York, NY (US); and Japanese Foundation for Cancer Research, Tokyo (JP)
Filed by Research Foundation of the City University of New York, New York, NY (US); and Japanese Foundation for Cancer Research, Tokyo (JP)
Filed on Jul. 2, 2021, as Appl. No. 17/366,950.
Application 17/366,950 is a continuation in part of application No. 16/436,331, filed on Jun. 10, 2019, abandoned.
Application 16/436,331 is a continuation in part of application No. 15/430,821, filed on Feb. 13, 2017, abandoned.
Claims priority of provisional application 62/293,852, filed on Feb. 11, 2016.
Prior Publication US 2021/0332401 A1, Oct. 28, 2021
Int. Cl. C12P 21/00 (2006.01); C12N 5/00 (2006.01)

CPC C12P 21/00 (2013.01) [C12N 5/0006 (2013.01)]

9 Claims

1. A method of enhanced extracellular vesicle production, the method comprising sequential steps of:

treating the plurality of cells with NapFFK(NDB)Yp at a concentration of at least 5 μM and less than 40 μM, wherein the NapFFK(NDB)Yp had been purified to a purity of at least 93%;

permitting the NapFFK(NDB)Yp to polymerize within cells in the plurality of cells due to an enzyme within the cells to form a modified cell culture; and

isolating a first quantity of extracellular vesicles from the modified cell culture, wherein the first quantity of extracellular vesicles is greater than a second quantity of extracellular vesicles isolated from a corresponding plurality of cells that was not treated with the NapFFK(NDB)Yp.