US 12,264,351 B2
Pisum sativum kaurene oxidase for high efficiency production of rebaudiosides
Gale Wichmann, Emeryville, CA (US); Aditi Khankhoje, Emeryville, CA (US); and Tina Mahatdejkul-Meadows, Emeryville, CA (US)
Assigned to AMYRIS, INC., Emeryville, CA (US)
Appl. No. 16/637,188
Filed by AMYRIS, INC., Emeryville, CA (US)
PCT Filed Aug. 10, 2018, PCT No. PCT/US2018/046359
§ 371(c)(1), (2) Date Feb. 6, 2020,
PCT Pub. No. WO2019/033064, PCT Pub. Date Feb. 14, 2019.
Application 16/637,188 is a continuation in part of application No. PCT/US2017/046637, filed on Aug. 11, 2017.
Claims priority of provisional application 62/544,718, filed on Aug. 11, 2017.
Prior Publication US 2020/0165651 A1, May 28, 2020
Int. Cl. C12N 9/20 (2006.01); C12N 15/81 (2006.01); C12P 7/40 (2006.01); C12P 19/56 (2006.01); C12N 1/18 (2006.01); C12R 1/865 (2006.01)
CPC C12P 19/56 (2013.01) [C12N 15/81 (2013.01); C12P 7/40 (2013.01); C12N 1/185 (2021.05); C12R 2001/865 (2021.05)] 14 Claims
 
1. A genetically modified Saccharomyces cerevisiae host cell that produces one or more steviol glycosides comprising a heterologous nucleic acid encoding a kaurene oxidase comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 1, wherein the genetically modified host cell is capable of converting kaurene to kaurenoic acid at an efficiency of greater than 30%, and wherein the genetically modified host cell produces one or more steviol glycosides,
wherein the genetically modified Saccharomyces cerevisiae host cell further comprises one or more heterologous nucleic acids, wherein the one or more heterologous nucleic acids encode enzymes comprising a geranylgeranylpyrophosphate synthase, a copalyl diphosphate synthase, an ent-kaurene synthase, a polypeptide having steviol synthase activity, and one or more polypeptides having UDP glycosyltransferase activity.