US 12,264,348 B2
Microorganism with knock-in at acetolactate decarboxylase gene locus
Ching Leang, Skokie, IL (US)
Assigned to LanzaTech, Inc., Skokie, IL (US)
Filed by LanzaTech, Inc., Skokie, IL (US)
Filed on May 2, 2023, as Appl. No. 18/310,636.
Application 18/310,636 is a division of application No. 17/303,677, filed on Jun. 4, 2021, granted, now 11,760,989.
Claims priority of provisional application 63/035,739, filed on Jun. 6, 2020.
Prior Publication US 2023/0348886 A1, Nov. 2, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/88 (2006.01); C12N 9/10 (2006.01); C12P 5/00 (2006.01); C12P 5/02 (2006.01); C12P 7/04 (2006.01); C12P 7/18 (2006.01); C12P 7/26 (2006.01); C12P 7/30 (2006.01); C12P 7/42 (2006.01); C12P 7/6409 (2022.01)
CPC C12N 9/88 (2013.01) [C12N 9/1029 (2013.01); C12N 9/13 (2013.01); C12P 5/007 (2013.01); C12P 5/026 (2013.01); C12P 7/04 (2013.01); C12P 7/18 (2013.01); C12P 7/26 (2013.01); C12P 7/30 (2013.01); C12P 7/42 (2013.01); C12P 7/6409 (2013.01); C12Y 208/03 (2013.01); C12Y 401/01001 (2013.01); C12Y 401/01005 (2013.01)] 21 Claims
 
1. A genetically engineered C1-fixing microorganism comprising knock-in of DNA at an acetolactate decarboxylase gene locus, wherein the microorganism comprises oscillations in metabolite production and gas consumption.