	US 12,264,342 B2
	Lachnospiraceae bacterium ND2006 CAS12A mutant genes and polypeptides encoded by same
Sarah Franz Beaudoin, Iowa City, IA (US); Michael Allen Collingwood, North Liberty, IA (US); and Christopher Anthony Vakulskas, North Liberty, IA (US)
Assigned to INTEGRATED DNA TECHNOLOGIES, INC., Coralville, IA (US)
Appl. No. 17/430,013
Filed by INTEGRATED DNA TECHNOLOGIES, INC., Coralville, IA (US)
PCT Filed Feb. 21, 2020, PCT No. PCT/US2020/019168 § 371(c)(1), (2) Date Aug. 11, 2021, PCT Pub. No. WO2020/172502, PCT Pub. Date Aug. 27, 2020.
Claims priority of provisional application 62/808,984, filed on Feb. 22, 2019.
Prior Publication US 2023/0040148 A1, Feb. 9, 2023
Int. Cl. C12N 9/22 (2006.01); C12N 15/11 (2006.01)

CPC C12N 9/22 (2013.01) [C12N 15/11 (2013.01); C12N 2310/20 (2017.05)]

8 Claims

1. An isolated mutant LbCas12a protein comprising

a single amino acid substitution mutation introduced into the wild-type LbCas12a protein comprising E795L (SEQ ID NO: 5).