CPC C12N 9/22 (2013.01) [C12N 9/1007 (2013.01); C12N 9/78 (2013.01); C12N 15/907 (2013.01); C07K 2319/71 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] | 35 Claims |
1. An isolated Streptococcus pyogenes Cas9 (SpCas9) protein, comprising an amino acid sequence that has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1, comprising one of the following sets of mutations: A61R/N1317R, G1104K/N1317R, A61R/G1104K, S55R/G1104K, S55R/A61R, S55R/N1317R, G1104K/T1314R, A61R/T1314R, T1314R/N1317R, S55R/T1314R, A61R/G1104R, G1104R/N1317R, S55R/G1104R, G1104R/T1314R, G366R/G1104K, S55R/G366R, A61R/G366R, G366R/N1317R, G366R/T1314R, G366R/G1104R, G1104K/A1285R, A61R/A1285R, A1285R/N1317R, S55R/A1285R, A1285R/T1314R, G1104R/A1285R, G366R/A1285R, G1104K/A1285K, A61R/A1285K, A1285K/N1317R, S55R/A1285K, A1285K/T1314R, G1104R/A1285K, G366R/A1285K, G1104K/D1332R, A61R/D1332R, D1332R/N1317R, S55R/D1332R, G1104R/D1332R, G366R/D1332R, A1285K/D1332R, A1285R/D1332R, A61R/G1104K/N1317R, A61R/L1111R, A61R/A1322R, A61R/L1111R/A1322R, G1104K/L1111R, G1104K/A1322R, G1104K/L1111R/A1322R, N1317R/L1111R, N1317R/A1322R, N1317R/L1111R/A1322R, A61R/N1317R/L1111R, A61R/N1317R/A1322R, A61R/N1317R/L1111R/A1322R, G1104K/N1317R/L1111R, G1104K/N1317R/A1322R, G1104K/N1317R/L1111R/A1322R, A61R/G1104K/L1111R, A61R/G1104K/A1322R, A61R/G1104K/L1111R/A1322R, S55R/L1111R, S55R/A1322R, S55R/L1111R/A1322R, G366R/L1111R, G366R/A1322R, G366R/L1111R/A1322R, A1285K/L1111R, A1285K/A1322R, or A1285K/L1111R/A1322R.
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