US 12,264,332 B2
Highly efficient gas permeable devices and methods for culturing cells
John R. Wilson, New Brighton, MN (US)
Assigned to Wilson Wolf Manufacturing, LLC, Saint Paul, MN (US)
Filed by Wilson Wolf Manufacturing, LLC, St. Paul, MN (US)
Filed on Nov. 2, 2021, as Appl. No. 17/516,773.
Application 17/516,773 is a continuation of application No. 15/643,621, filed on Jul. 7, 2017, granted, now 11,377,635, issued on Jul. 5, 2022.
Application 15/643,621 is a continuation of application No. 14/321,933, filed on Jul. 2, 2014, granted, now 9,732,317, issued on Aug. 15, 2017.
Application 14/321,933 is a continuation of application No. 11/952,848, filed on Dec. 7, 2007, granted, now 8,809,044, issued on Aug. 19, 2014.
Claims priority of provisional application 60/873,347, filed on Dec. 7, 2006.
Prior Publication US 2022/0049215 A1, Feb. 17, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/00 (2006.01); C12M 1/04 (2006.01); C12M 1/24 (2006.01); C12N 5/071 (2010.01)
CPC C12N 5/0602 (2013.01) [C12M 23/08 (2013.01); C12M 23/24 (2013.01); C12M 23/34 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A cell growth apparatus, comprising:
an enclosure that does not include equipment for pumping gas or medium through the enclosure, the enclosure bounded by a liquid impermeable and gas permeable wall defining an interior volume capable of being filled with liquid and an exterior surface configured for contact with incubator gas, the wall forming more than one shelf with an interior surface for cells to reside upon, the interior surface of each shelf having and an opposing parallel surface located a distance away from it, each shelf being gas permeable, the shelves being located one above the other when the shelves are in a horizontal position, and the enclosure geometry having a space for incubator gas to exist below each shelf, and each shelf having an exterior surface in contact with projections that define a space for incubator gas to make contact with the exterior surface, wherein the projections are arranged in a bed of nails pattern and maintain each shelf in a horizontal position during cell culture, the enclosure is capable of being completely filled with media for optimal cell-nutrient exchange while still allowing ambient gas to make contact with a portion of the outside surface of each shelf.