US 12,264,318 B2
Ribosome-mediated incorporation of peptides and peptidomimetics
Sidney Hecht, Phoenix, AZ (US); Larisa (Liza) Dedkova, Scottsdale, AZ (US); Rumit Maini, Tempe, AZ (US); Sandipan Roy Chowdhury, Tempe, AZ (US); and Rakesh Paul, Baltimore, MD (US)
Assigned to Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US)
Filed by Sidney Hecht, Phoenix, AZ (US); Larisa (Liza) Dedkova, Scottsdale, AZ (US); Rumit Maini, Tempe, AZ (US); Sandipan Roy Chowdhury, Tempe, AZ (US); and Rakesh Paul, Baltimore, MD (US)
Filed on Jul. 19, 2022, as Appl. No. 17/813,400.
Application 16/915,362 is a division of application No. 15/545,275, granted, now 10,745,705, previously published as PCT/US2016/014548, filed on Jan. 22, 2016.
Application 17/813,400 is a continuation of application No. 16/915,362, filed on Jun. 29, 2020, granted, now 11,434,492.
Claims priority of provisional application 62/106,958, filed on Jan. 23, 2015.
Prior Publication US 2023/0046194 A1, Feb. 16, 2023
Int. Cl. C12N 15/67 (2006.01); C07D 263/34 (2006.01); C07D 277/593 (2006.01); C12N 9/06 (2006.01); C12N 15/11 (2006.01)
CPC C12N 15/67 (2013.01) [C07D 263/34 (2013.01); C07D 277/593 (2013.01); C12N 9/003 (2013.01); C12N 15/11 (2013.01); C12Y 105/01003 (2013.01)] 16 Claims
 
1. An Escherichia coli (E. coli) bacterial ribosome comprising a modified 23S ribosomal RNA sequence comprising modifications as compared to a wild-type counterpart, wherein the modifications consist of a first modified region (Region 1) and a second modified region (Region 2);
(a) if the modification in Region 1 consists of UGCGUGG from position 2057-2063, the modification in Region 2 is selected from the group consisting of ACGAAG, CGCACG, CUAUGU, CGCAAU, or CUACAG from position 2502-2507;
(b) if the modification in Region 1 consists of AGCGUGA from position 2057-2063, the modification in Region 2 is selected from the group consisting of CUGCGU, UGGCAG, AUCAGG, or AUCCGA from position 2502-2507;
wherein the wild-type counterpart DNA sequence of the first unmodified region (Region 1) consists of the sequence GAAAGAC from position 2057 to 2063;
wherein the wild-type counterpart DNA sequence of the second unmodified region (Region 2) consists of the sequence GAUGUC from position 2502-2507; and
wherein the 23S ribosomal RNA is expressed from an engineered rrnB operon.