US 12,264,312 B2
Method for extracting nucleic acids
Alain Laurent, Grenoble (FR); Arnaud Burr, Saint Martin Le Vinoux (FR); and Ali Laayoun, La Frette (FR)
Assigned to BIOMÉRIEUX, Marcy l'Etoile (FR)
Appl. No. 16/979,565
Filed by BIOMÉRIEUX, Marcy L'Etoile (FR)
PCT Filed Mar. 15, 2019, PCT No. PCT/FR2019/050591
§ 371(c)(1), (2) Date Sep. 10, 2020,
PCT Pub. No. WO2019/175518, PCT Pub. Date Sep. 19, 2019.
Claims priority of application No. 1852257 (FR), filed on Mar. 16, 2018.
Prior Publication US 2021/0009989 A1, Jan. 14, 2021
Int. Cl. C12N 15/10 (2006.01)
CPC C12N 15/1006 (2013.01) 19 Claims
 
1. A method for extracting nucleic acids from a sample comprising proteins and/or polysaccharides, said method comprising:
a step of treating the sample with:
a reagent for masking the amine functional groups of the proteins and/or polysaccharides of the sample, which is selected from acylating agents in concentrations between 0.1 and 1.8 M, and/or
a reagent for masking the carboxylic acid functional groups of the proteins and/or polysaccharides of the sample, which is selected from the group consisting of amines, alcohols, and thiols, in combination with a coupling agent; and
a step of capturing nucleic acids by placing the treated sample in contact with a suitable solid support,
wherein the nucleic acids are not modified by the masking reagent.