US 12,264,311 B2
Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip
Takamasa Kouno, Osaka (JP); Nobuhiko Inui, Saitama (JP); Tsutomu Nakamura, Osaka (JP); Tomoya Sasaki, Saitama (JP); Sou Yamaguchi, Tokyo (JP); and Katsura Uchida, Tokyo (JP)
Assigned to SEKISUI CHEMICAL CO., LTD., Osaka (JP); and SEKISUI MEDICAL CO., LTD., Tokyo (JP)
Appl. No. 17/422,602
Filed by SEKISUI CHEMICAL CO., LTD., Osaka (JP); and SEKISUI MEDICAL CO., LTD., Tokyo (JP)
PCT Filed Jan. 17, 2020, PCT No. PCT/JP2020/001461
§ 371(c)(1), (2) Date Jul. 13, 2021,
PCT Pub. No. WO2020/153248, PCT Pub. Date Jul. 30, 2020.
Claims priority of application No. 2019-011186 (JP), filed on Jan. 25, 2019; application No. 2019-028014 (JP), filed on Feb. 20, 2019; application No. 2019-067480 (JP), filed on Mar. 29, 2019; and application No. 2019-183676 (JP), filed on Oct. 4, 2019.
Prior Publication US 2022/0090048 A1, Mar. 24, 2022
Int. Cl. C12N 15/10 (2006.01); C12Q 1/6806 (2018.01)
CPC C12N 15/1003 (2013.01) [C12Q 1/6806 (2013.01)] 7 Claims
 
1. A nucleic acid isolation method, comprising steps of:
mixing a specimen containing a nucleic acid with an extraction solution to disperse the nucleic acid in the extraction solution;
bringing the extraction solution containing the nucleic acid into contact with an anionic adsorbent;
bringing a washing solution into contact with the anionic adsorbent; and
bringing a collection solution into contact with the anionic adsorbent,
the extraction solution containing a protein denaturant,
the washing solution containing a basic compound and having a pH value equal to or less than a pKa value of a conjugate acid of the basic compound,
the basic compound containing at least one selected from the group consisting of melamine, and 1,4-diazabicyclo[2.2.2]octane, and
the collection solution having a pH value equal to or more than the pKa value of the conjugate acid of the basic compound.