US 12,264,308 B2
System and methods for immune cells expansion and activation in large scale
Lior Raviv, Kfar Monash (IL); Shirley Bachar, Petach Tikva (IL); and Dorina Roberman, Nahariya (IL)
Assigned to PLURI BIOTECH LTD., Haifa (IL)
Filed by PLURI BIOTECH LTD., Haifa (IL)
Filed on Mar. 2, 2024, as Appl. No. 18/593,868.
Application 18/593,868 is a division of application No. 18/200,942, filed on May 23, 2023, granted, now 11,939,562.
Prior Publication US 2024/0392225 A1, Nov. 28, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12N 5/078 (2010.01)
CPC C12M 25/14 (2013.01) [C12M 23/02 (2013.01); C12M 23/20 (2013.01); C12M 25/18 (2013.01); C12N 5/0634 (2013.01); C12N 2513/00 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A three-dimensional (3D) bioreactor for large scale expansion of immune cells, the 3D bioreactor comprising:
at least one packed bed chamber;
at least one porous scaffold surrounded by said at least one packed bed chamber, wherein said at least one porous scaffold is coated with one or more extra cellular matrix protein (ECM) and coated or linked with at least one immune cell activator;
at least one container surrounding said at least one packed bed chamber;
a fluid media having at least one population of immune cells suspended in said fluid media contained in said at least one container; and
at least one gene modifying agent spiked into the fluid media for genetically modifying immune cells inside the 3D bioreactor;
wherein said fluid media having at least one population of immune cells suspended in said fluid media flows through said packed bed chamber and said at least one porous scaffold coated with one or more ECM causing said expanded immune cells to be further activated within said at least one packed bed chamber upon exposure of said population of immune cells to said at least one porous scaffold coated with one or more ECM coated or linked with at least one immune cell activator.