US 12,264,307 B2
Culture container, method for culturing lymphocytes, culture-container production method, and solid-phasing apparatus
Takahiko Totani, Kanagawa (JP); Satoshi Tanaka, Kanagawa (JP); Takeshi Aihara, Kanagawa (JP); and Yoichi Ishizaki, Kanagawa (JP)
Assigned to TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Filed by TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Filed on Jan. 6, 2022, as Appl. No. 17/569,943.
Application 17/569,943 is a division of application No. 15/184,649, filed on Jun. 16, 2016, granted, now 11,884,907.
Application 15/184,649 is a continuation of application No. PCT/JP2014/006252, filed on Dec. 16, 2014.
Claims priority of application No. 2013-261181 (JP), filed on Dec. 18, 2013; and application No. 2013-267082 (JP), filed on Dec. 25, 2013.
Prior Publication US 2022/0127555 A1, Apr. 28, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/07 (2010.01); C07K 17/00 (2006.01); C12M 1/00 (2006.01); C12M 1/04 (2006.01); C12M 1/12 (2006.01); C12N 5/078 (2010.01); C12N 5/0783 (2010.01)
CPC C12M 25/00 (2013.01) [C12M 23/14 (2013.01); C12M 23/20 (2013.01); C12M 23/24 (2013.01); C12M 25/06 (2013.01); C12N 5/0634 (2013.01); C12N 5/0636 (2013.01); C12N 2501/515 (2013.01); C12N 2513/00 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01)] 6 Claims
 
1. A method for culturing lymphocytes by using a culture container for culturing lymphocytes comprising an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm2, comprising the steps of:
an activation step in which lymphocytes and a culture liquid are sealed in the culture container, and the culture container is arranged such that the immobilized surface in the culture container becomes a bottom surface, thereby to activate lymphocytes; and
a proliferation step in which the culture container is inverted and arranged such that the non-immobilized surface in the culture container becomes a bottom surface, thereby to proliferate lymphocytes.