US 12,264,305 B2
Devices and methods for isolating a migratory cell population
Young-tae Kim, Arlington, TX (US); Loan Bui, Arlington, TX (US); James Battiste, Edmond, OK (US); Xue Cai, Edmond, OK (US); and Michael Sughrue, Sydney (AU)
Assigned to BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, Austin, TX (US); and THE BOARD OF REGENTS OF THE UNIVERSITY OF OKLAHOMA, Norman, OK (US)
Filed by Board of Regents, The University of Texas System, Austin, TX (US); and THE BOARD OF REGENTS OF THE UNIVERSITY OF OKLAHOMA, Norman, OK (US)
Filed on Sep. 18, 2019, as Appl. No. 16/574,587.
Claims priority of provisional application 62/732,806, filed on Sep. 18, 2018.
Prior Publication US 2020/0087606 A1, Mar. 19, 2020
Int. Cl. C12M 3/06 (2006.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01); C12N 5/09 (2010.01)
CPC C12M 23/16 (2013.01) [C12M 29/04 (2013.01); C12M 33/00 (2013.01); C12N 5/0693 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method of isolating a migratory cell population from a mixed population of migratory cells and non-migratory cells, the method comprising:
placing the mixed population of migratory cells and non-migratory cells in contact with one or more microchannels;
commencing an incubation period to allow the migratory cells to migrate into the microchannels;
isolating the migratory cell population within the microchannels following the incubation period by attaching the migratory cells to the surface of the microchannels; and
collecting the migratory cell population from the microchannels,
wherein the one or more microchannels have an average size in a proliferation direction of the migratory cells that is less than a minimum proliferation space of the migratory cells along the proliferation direction, and
wherein at least 500 migratory cells are isolatable within the microchannels at the same time by carrying out the steps as a single method of placing the mixed population of migratory cells and non-migratory cells in contact with one or more microchannels, commencing an incubation period to allow the migratory cells to migrate into the microchannels, isolating the migratory cell population within the microchannels following the incubation period by attaching the migratory cells to the surface of the microchannels, and collecting the migratory cell population from the microchannels only once.