CPC C12F 3/10 (2013.01) [A23K 10/38 (2016.05); B01D 21/0084 (2013.01); B01D 21/01 (2013.01); C02F 1/24 (2013.01); C02F 1/56 (2013.01); C12P 7/06 (2013.01); C02F 2101/32 (2013.01); C02F 2101/38 (2013.01); C02F 2103/32 (2013.01)] | 7 Claims |
1. A method of recovering protein from stillage produced in an ethanol production process, the method comprising:
treating stillage comprising oil, protein, and water upstream of a separation, concentration or evaporation step with about 50 ppm to about 500 ppm of a coagulant comprising poly(diallyldimethylammonium chloride) (polyDADMAC), epichlorohydrin-diethylamine, or a combination thereof, and about 50 ppm to about 500 ppm of a polymer comprising a monomer unit derived from a monomer selected from 2-acrylamido-2-methylpropane sulfonic acid (“AMPS”), 2-acrylamido-2-methylbutane sulfonic acid (“AMBS”), [2-methyl-2-[(1-oxo-2-propenyl)amino |propyl]-phosphonic acid, methacrylic acid, acrylic acid, salts thereof, and combinations thereof, to produce a treated stillage comprising solids which include at least a portion of the oil and protein; and subjecting the treated stillage to a solid/liquid separation process, to produce a clarified stillage comprising a clarified aqueous phase and a separated solids phase, wherein the separated solids phase comprises at least a portion of the protein from the stillage,
wherein the stillage is whole stillage or thin stillage and the separated solids phase is in the form of a float layer and
the method further comprising separating at least a portion of the oil from the float layer to produce a de-oiled float layer by treating the float layer with an oil recovery agent, comprising a blend of 75-95% castor oil ethoxylate, 5-15% hydrophobic precipitated silica, 10-30% vegetable oil, and <10% propylene glycol.
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