US 12,264,113 B2
Methods for carbon capture and increasing yield of plants
Ray Riley, St. Paul, MN (US); Andres Reyes, St. Paul, MN (US); Abed Chaudhury, Orange (AU); Suresh Subashchandrabose, Orange (AU); Ahsanul Haque, Orange (AU); Neeraj Purushotham, Orange (AU); Raghvendra Sharma, Orange (AU); Tegan Nock, Orange (AU); Gyongyver Korosi, Orange (AU); Brooke Bruning, Orange (AU); Grace Scott, Orange (AU); and Venkatachalam Lakshmanan, St. Paul, MN (US)
Assigned to Loam Bio Pty Ltd, New South Wales (AU)
Appl. No. 18/565,464
Filed by Loam Bio Pty Ltd, Orange (AU)
PCT Filed May 30, 2022, PCT No. PCT/US2022/031487
§ 371(c)(1), (2) Date Nov. 29, 2023,
PCT Pub. No. WO2022/256275, PCT Pub. Date Dec. 8, 2022.
Claims priority of provisional application 63/195,109, filed on May 31, 2021.
Claims priority of provisional application 63/195,110, filed on May 31, 2021.
Claims priority of provisional application 63/244,474, filed on Sep. 15, 2021.
Prior Publication US 2024/0251803 A1, Aug. 1, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C05F 11/08 (2006.01); A01H 3/00 (2006.01); A01N 63/30 (2020.01); A01P 21/00 (2006.01); C12N 1/14 (2006.01); C12R 1/645 (2006.01); C12R 1/885 (2006.01)
CPC C05F 11/08 (2013.01) [A01H 3/00 (2013.01); A01N 63/30 (2020.01); A01P 21/00 (2021.08); C12N 1/145 (2021.05); C12R 2001/645 (2021.05); C12R 2001/885 (2021.05)] 14 Claims
 
1. A method of increasing organic carbon in a soil, comprising:
inoculating the soil and/or a plant growing in the soil with one or more fungal strains having a nuclear ribosomal internal transcribed spacer 2 (ITS2) sequence that is at least 90% identical to the nucleotide sequence of SEQ ID No: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11. 12, 13, 14, 15, 16, 17, 18, or 19;
wherein the one or more fungal strains are selected from the group consisting of Acrocalymma vagum DMTR-CTR-11556 (NMI Accession No. V22/006357), Leptodontidium orchidicola DMTR-CTR-4873 (NMI Accession No. V22/003497), Periconia sp. DMTR-CTR-6649 (NMI Accession No. V22/006356), Periconia macrospinosa DMTR-CTR-US-125 (ATCC Accession No. PTA-127300), Periconia macrospinosa DMTR-CTR-1852 (NMI Accession No. V22/006358), Phaeosphaeria luctuosa/vagans DMTR-CTR-3044 (NMI Accession No. V22/006355), Thozetella nivea DMTR-CTR-2359 (NMI Accession No. V22/003496), Trichoderma hamatum DMTR-CTR-US-73 (ATCC Accession No. PTA-127301), Trichoderma longipile/spirale DMTR-CTR-1291 (NMI Accession No. V22/006354), and a combination thereof; and
the one or more fungal strains are inoculated in an amount effective to increase organic carbon in the soil compared to a non-inoculated control soil.