US 12,264,092 B2
Constructed wetland system enhanced by immobilized laccase
Zhen Hu, Jinan (CN); Xinhan Chen, Jinan (CN); Jian Zhang, Jinan (CN); Huijun Xie, Jinan (CN); and Shuang Liang, Jinan (CN)
Assigned to SHANDONG UNIVERSITY, Jinan (CN)
Appl. No. 17/791,444
Filed by SHANDONG UNIVERSITY, Shandong (CN)
PCT Filed May 11, 2021, PCT No. PCT/CN2021/092936
§ 371(c)(1), (2) Date Jul. 7, 2022,
PCT Pub. No. WO2021/238640, PCT Pub. Date Dec. 2, 2021.
Claims priority of application No. 202010474725.X (CN), filed on May 29, 2020.
Prior Publication US 2023/0048966 A1, Feb. 16, 2023
Int. Cl. C02F 3/32 (2023.01); C02F 3/34 (2023.01); C02F 101/30 (2006.01); C02F 103/26 (2006.01)
CPC C02F 3/342 (2013.01) [C02F 3/32 (2013.01); C02F 2101/30 (2013.01); C02F 2103/26 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A constructed wetland system, comprising:
a matrix layer comprising a vertically oriented laccase catalyst module and a laterally adjacent vertically oriented gravel module;
wetland plants growing on the matrix layer;
a water distribution system arranged on two laterally opposite sides of the matrix layer; and
a perforated water inlet pipe arranged in the matrix layer and connected to the water distribution system,
wherein the laccase catalyst module comprises laccasse catalyst consisting of
8-12 g laccase natural phenol medium extract being an active substance,
0.2-0.8 g sodium alginate being a carrier,
0.05-0.5 g laccase being an active substance,
0.2-1 g chitosan being a modifier,
1-1.25 g acetic acid being a modifier, and
0.1-0.6 g of calcium chloride being a carrier,
wherein the laccase natural phenol medium extract is obtained by passing a shaken mixture of deionized water and a phenol extract of soybean meal through a cellulose acetate membrane.