US 12,263,489 B2
Manufacturing method of the operation pipe
Masamitsu Shikata, Kyoto (JP); Ayaka Minamimoto, Kyoto (JP); Akira Muramatsu, Kyoto (JP); Masaki Kanai, Kyoto (JP); Seiya Fujiwara, Kyoto (JP); Tetsuo Ohashi, Kyoto (JP); and Hiroyuki Jikuya, Kyoto (JP)
Assigned to SHIMADZU CORPORATION, Kyoto (JP)
Filed by SHIMADZU CORPORATION, Kyoto (JP)
Filed on Jun. 14, 2022, as Appl. No. 17/840,568.
Application 17/840,568 is a continuation of application No. 16/782,064, filed on Feb. 5, 2020, abandoned.
Claims priority of application No. 2019-043228 (JP), filed on Mar. 9, 2019.
Prior Publication US 2022/0355310 A1, Nov. 10, 2022
Int. Cl. B03C 1/12 (2006.01); B01D 15/38 (2006.01); B01L 3/00 (2006.01); B01L 9/06 (2006.01); B03C 1/01 (2006.01); B03C 1/03 (2006.01); B03C 1/033 (2006.01); B03C 1/28 (2006.01); B03C 1/30 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/6865 (2018.01)
CPC B03C 1/12 (2013.01) [B01D 15/3819 (2013.01); B01D 15/3885 (2013.01); B01L 3/5085 (2013.01); B01L 9/06 (2013.01); B03C 1/01 (2013.01); B03C 1/0332 (2013.01); B03C 1/0335 (2013.01); B03C 1/288 (2013.01); B03C 1/30 (2013.01); C12N 15/1013 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6865 (2013.01); B01L 3/5025 (2013.01); B01L 2200/0647 (2013.01); B01L 2400/043 (2013.01); B03C 2201/18 (2013.01); B03C 2201/26 (2013.01); C12Q 2563/107 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A manufacturing method of an operation pipe for operating target components, comprising:
providing a hollow pipe having a closable open end for supplying a sample containing the target components on one side and a closed end on the other side, and having an operation pipe portion on the open end side and a recovery pipe portion on the closed end side;
filling a recovery medium in the recovery pipe portion so that a first gel layer and a first aqueous liquid layer which is in contact with the closed end are multi-layered, wherein the first aqueous liquid layer in contact with the closed end has a predetermined volume, a layer length of the first aqueous liquid layer in contact with the closed end varies with variations in an inner diameter of the hollow pipe or a length of the hollow pipe, and a volume of the first aqueous liquid layer in contact with the closed end is 50 μL-300 μL, and a layer length of the first gel layer is determined by a length in the longitudinal direction of the hollow pipe;
filling an operation medium in the operation pipe portion so that second gel layers and second aqueous liquid layers are alternately multi-layered in the longitudinal direction of the hollow pipe, wherein a layer length of each of the second gel layers and a layer length of each of the second aqueous liquid layers are determined by the length in the longitudinal direction of the hollow pipe.