US 12,263,485 B2
Substrate for nucleic acid amplification, and method for manufacturing same
Oh Seok Kwon, Daejeon (KR); Tai Hwan Ha, Daejeon (KR); Kyung Ho Kim, Daejeon (KR); Jin Yeong Kim, Daejeon (KR); Seon Joo Park, Daejeon (KR); and Seong Eun Seo, Daejeon (KR)
Assigned to KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY, Daejeon (KR)
Appl. No. 17/259,689
Filed by KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY, Daejeon (KR)
PCT Filed Jul. 12, 2019, PCT No. PCT/KR2019/008671
§ 371(c)(1), (2) Date Jan. 12, 2021,
PCT Pub. No. WO2020/013668, PCT Pub. Date Jan. 16, 2020.
Claims priority of application No. 10-2018-0081089 (KR), filed on Jul. 12, 2018.
Prior Publication US 2021/0229104 A1, Jul. 29, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 7/00 (2006.01); B01J 19/00 (2006.01)
CPC B01L 7/52 (2013.01) [B01J 19/0046 (2013.01); B01J 2219/0031 (2013.01); B01J 2219/00497 (2013.01); B01J 2219/00722 (2013.01); B01L 2200/0663 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/123 (2013.01); B01L 2300/16 (2013.01); B01L 2300/1861 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A substrate for nucleic acid amplification, used in light-based polymerase chain reaction (PCR) using thermal energy produced by the irradiation of light energy from light sources, comprising:
a transparent matrix;
a micro-patterned metal layer formed on the transparent matrix;
an N-heterocyclic carbene compound having one end bonded to the surface of the micro-patterned metal layer; and
at least one pair of primers immobilized on the other end of the N-heterocyclic carbene compound,
wherein the N-heterocyclic carbene compound is represented by the following Chemical Formula 1 or 2:

OG Complex Work Unit Chemistry
in Chemical Formulae 1 and 2,
R1, R2, R5 and R6 are the same or different, and are each independently hydrogen, an alkyl group of 1 to 20 carbon atoms, a cycloalkyl group of 3 to 20 carbon atoms, an aryl group of 6 to 30 carbon atoms or a heteroaryl group of 2 to 30 carbon atoms,
R3, R4, R7, R8, R9 and R10 are the same or different, and are each independently, hydrogen, an alkyl group of 1 to 20 carbon atoms, a cycloalkyl group of 3 to 20 carbon atoms, an aryl group of 6 to 30 carbon atoms, a heteroaryl group of 2 to 30 carbon atoms or a structure represented by the following Chemical Formula 3, where adjacent two or more substituents among R7 to R10 are bonded to form a hydrocarbon ring,
at least one of R3 and R4 is the structure represented by the following Chemical Formula 3, and
at least one of R7 to R10 is substituted with the structure represented by the following Chemical Formula 3, or in case where adjacent two or more substituents among R7 to R10 are bonded to form a hydrocarbon ring, at least one of carbon atoms forming the hydrocarbon ring is substituted with the structure represented by the following Chemical Formula 3:

OG Complex Work Unit Chemistry
in Chemical Formula 3,
n is a repeating number of a unit in parenthesis and an integer of 1 to 30, and
A is a nitrogen (N)-containing alkyl group of 1 to 20 carbon atoms or a nitrogen (N)-containing heteroaryl group of 2 to 30 carbon atoms.