| CPC B01L 3/502761 (2013.01) [G01N 33/54326 (2013.01); G01N 33/582 (2013.01); G01N 35/0098 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0681 (2013.01); B01L 2400/086 (2013.01)] | 16 Claims |
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1. An analysis device comprising:
a microchip;
a first upper magnetic force application part disposed above the microchip and a first lower magnetic force application part disposed below the microchip;
a second upper magnetic force application part disposed above the microchip and a second lower magnetic force application part disposed below the microchip; and
a sensor,
wherein the microchip comprises:
a body including an upper portion and a lower portion; and
a plurality of internal channels formed between the upper portion and the lower portion,
wherein the plurality of internal channels comprise:
an input channel having an input channel upper surface and an input channel lower surface and configured to receive an assay sample including target antigens and injected through a through hole penetrating the upper portion of the body,
a reaction channel having a reaction channel upper surface and a reaction channel lower surface and configured to be in fluid communication with the input channel and including magnetic particle-first antibody complexes to be subjected to a first antigen-antibody reaction with the target antigens in the assay sample flowed from the input channel, wherein the magnetic particle-first antibody complexes include magnetic particles and first antibodies, and the magnetic particle-first antibody complexes and the target antigens form immune complexes through the first antigen-antibody reaction, and
a detection channel having a detection channel upper surface and a detection channel lower surface and configured to be in fluid communication with the reaction channel and including second antibodies to be subjected to a second antigen-antibody reaction with the immune complexes flowed from the reaction channel,
wherein the input channel upper surface of the input channel, the reaction channel upper surface of the reaction channel and the detection channel upper surface of the detection channel constitute the upper portion of the body,
wherein the input channel lower surface of the input channel, the reaction channel lower surface of the reaction channel and the detection channel lower surface of the detection channel constitute the lower portion of the body,
wherein the reaction channel lower surface of the reaction channel has a higher height than the input channel lower surface of the input channel,
wherein the detection channel has a longer length than each of a length of the input channel and a length of the reaction channel,
wherein the detection channel has a narrower width than each of a width of the input channel and a width of the reaction channel,
wherein the microchip further comprises flow control pillars for controlling flow of the assay sample, and
wherein the flow control pillars comprise a control pillar attached to the reaction channel upper surface of the reaction channel for physically regulating flow of the magnetic particle-first antibody complexes and a control pillar attached to the detection channel upper surface of the detection channel,
wherein the sensor is configured to count the number of the target antigens in the assay sample,
wherein each of the first upper magnetic force application part and the first lower magnetic force application part is disposed at a position corresponding to the reaction channel,
wherein each of the first upper magnetic force application part and the first lower magnetic force application part comprises a core magnetic material and a plurality of magnetic material pairs protruding from the core magnetic material and surrounding the core magnetic material,
wherein the plurality of magnetic material pairs include:
a first magnetic material pair protruding from the core magnetic material,
a second magnetic material pair protruding from the core magnetic material and adjacent to the first magnetic material pair, and
a third magnetic material pair protruding from the core magnetic material and adjacent to the second magnetic material pair, and
wherein the device is configured to sequentially apply electromagnetic force to the first magnetic material pair, the second magnetic material pair and the third magnetic material pair so that the magnetic particle-first antibody complexes rotate in the reaction channel,
wherein each of the second upper magnetic force application part and the second lower magnetic force application part is disposed at a position corresponding to the detection channel, and
wherein the device is further configured to control application of electromagnetic force to the second upper magnetic force application part and the second lower magnetic force application part to control flow of the magnetic particle-first antibody complexes.
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