US 12,263,479 B2
Array microfluidic chip and method of antibiotic susceptibility testing
Wen-Bin Lee, Kaohsiung (TW); and Shu-Hsien Liao, New Taipei (TW)
Assigned to MEDFLUID CO., LTD., Taipei (TW)
Filed by MEDFLUID CO., LTD., Taipei (TW)
Filed on Sep. 30, 2021, as Appl. No. 17/490,014.
Claims priority of application No. 110112449 (TW), filed on Apr. 6, 2021.
Prior Publication US 2022/0314222 A1, Oct. 6, 2022
Int. Cl. B01L 3/00 (2006.01); G01N 33/50 (2006.01)
CPC B01L 3/502723 (2013.01) [G01N 33/5008 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/161 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An array microfluidic chip, comprising:
a chip mainbody, comprising:
a sample loading well disposed on one side portion of the chip mainbody;
a plurality of reaction wells respectively pipe-connected to the sample loading well, wherein the reaction wells are arranged in an array form; and
an independent well disposed separately from the sample loading well;
a transparent hydrophilic membrane disposed on the chip mainbody and covering the reaction wells, wherein the transparent hydrophilic membrane comprises:
a plurality of air pores respectively connected to one of the reaction wells;
a first opening correspondingly connected to the sample loading well; and
a second opening correspondingly connected to the independent well; and
a covering sheet disposed on the transparent hydrophilic membrane and covering the air pores, wherein the covering sheet comprises:
an adhesive element disposed on one surface of the covering sheet and located between the covering sheet and the transparent hydrophilic membrane, wherein the adhesive element is arranged in a ring shape along an outer edge portion of the covering sheet; and
a vent hole, wherein the covering sheet, the adhesive element and the transparent hydrophilic membrane are stacked to form a vent space, the vent space is connected to an external space of the array microfluidic chip through the vent hole, and the reaction wells are connected to the vent space through the air pores.